Handbook of Practical Immunohistochemistry
In a conceptually current, quick-reference, Question & Answer format, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions provides standardization of the immunostaining process for each antibody and for each staining panel. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 5,000 tumors to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Chapters are presented in a unique Question and Answer format. One table/IHC panel is provided to address each question. A concise explanatory note follows each table/panel to avoid diagnostic pitfalls. Website links are provided throughout to update the massive information in this field, providing the most current knowledge and the potential for live expert consultation. All chapters are written by nationally/internationally recognized experts in the related area ensuring authority and excellence. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.
Inhoud1. Quality management and regulation Jeffrey Prichard 2. Technique and troubleshooting of antibody testing Fan Lin and Jianhui Shi 3. Overview of automated immunohistochemistry Jeff Prichard, Angela Bitting and Joe Myers 4. Automated staining - Dako Perspective Ole F. Rasmussen and Andreas Schonau 5. Automated staining - Ventana Perspective Angela Sattler 6. Tissue microarray Myra Wilkerson and Erin Powell 7. Unknown primary/undifferentiated neoplasms in surgical and cytologic specimens Fan Lin and Haiyan Liu 8. Exfoliative cytopathology Steven Meschter and Jan F. Silverman 9. Predictive markers of breast cancer: ER, PR and Her-2/neu Jeffrey Prichard 10. Central and peripheral nerve system tumors Hueizhi (Hope) Wu, Conrad Schuerch, and Douglas C. Miller 11. Thyroid and parathyroid gland Haiyan Liu, Fan Lin and Ronald A. DeLellis 12. Adrenal gland Hanna Kaspar 13. Salivary gland and other head and neck structures Conrad Schuerch 14. Lung, pleura, and mediastinum Kai Zhang and Phillip Cagle 15. Breast Haiyan Liu 16. Uterus Hanna Kaspar 17. Ovary Jeffrey Prichard, Haiyan Liu and Myra Wilkerson 18. Prostate gland Haiyan Liu, Fan Lin and Qihui (Jim) Zhai 19. Urinary bladder Myra Wilkerson 20. Kidney Fan Lin and Ximing J. Yang 21. Testis and paratesticular tissues Myra Wilkerson 22. Pancreas and ampullae Fan Lin and Hanlin L. Wang 23. Liver, bile ducts and gallbladder Jeffrey Prichard and Fan Lin 24. Upper gastrointestinal tract Jinhong Li and Fan Lin 25. Lower gastrointestinal tract and microsatellite instability (MSI) Jinhong Li and Fan Lin 26. Soft tissue and bone tumors Shaobo Zhu and Markku Miettinen 27. Lymph node Xiaohong (Mary) Zhang and Nadine S. Aguilera 28. Bone Marrow R. Patrick Dorion and Xiaohong (Mary) Zhang 29. Infectious diseases Dirk M. Elston, Lawrence E. Gibson and Heinz Kutzner 30. Skin Tammie Ferringer 31. Application of Direct Immunofluorescence for skin and mucosal biopsies William B. Tyler 32. In situ hybridization in surgical and cytologic specimens Hong Yin and Barbara Paynton 33. Appendices - Antibody information and protocols (Appendix I using Dako System; Appendix II using Ventana System) Fan Lin, Jianhui Shi, Angela Bitting, and Jeffrey Prichard